Journal: Epigenetics & Chromatin

Article Title: The pattern of histone H3 epigenetic posttranslational modifications is regulated by the VRK1 chromatin kinase

doi: 10.1186/s13072-023-00494-7

Figure Lengend Snippet: Interaction of SETDB1 (KMT1E) and KDM with VRK1. A Left: reciprocal interaction of SETDB1 with endogenous VRK1. Right: reciprocal interaction of SETDB1-Flag (KMT1E) with transfected VRK1-HA. B Left: reciprocal interaction of KDM4A (JMJD2A)-HA (top) or KDM3A (JMJD1A)-V5 (bottom) with endogenous VRK1. Right: reciprocal interaction of JMJD2A-HA (top) or KDM3A-V5 (bottom) with transfected VRK1-HA. Transfections were performed in HEK293T cells

Article Snippet: KDM4A (JMJD2A) , pCMV , HA , Mammalian , Addgene, #24180.

Techniques: Transfection

Journal: Epigenetics & Chromatin

Article Title: The pattern of histone H3 epigenetic posttranslational modifications is regulated by the VRK1 chromatin kinase

doi: 10.1186/s13072-023-00494-7

Figure Lengend Snippet: Inhibitors of histone PTMs

Article Snippet: KDM4A (JMJD2A) , pCMV , HA , Mammalian , Addgene, #24180.

Techniques: Concentration Assay

Journal: Epigenetics & Chromatin

Article Title: The pattern of histone H3 epigenetic posttranslational modifications is regulated by the VRK1 chromatin kinase

doi: 10.1186/s13072-023-00494-7

Figure Lengend Snippet: Plasmids

Article Snippet: KDM4A (JMJD2A) , pCMV , HA , Mammalian , Addgene, #24180.

Techniques: Plasmid Preparation, Expressing

Journal: Cancer Cell International

Article Title: KLF8 overexpression promotes the growth of human lung cancer cells by promoting the expression of JMJD2A

doi: 10.1186/s12935-019-0970-3

Figure Lengend Snippet: JMJD2A is involved in the regulation of the cell cycle and cell growth by KLF8. a The mRNA level of JMJD2A is overexpressed in human lung cancer cell lines (A549, H1975, H1299, H460, and H520) compared with normal lung epithelial cell line (BEAS-2B). *p < 0.05 and *** p < 0.001. n = 3 in each group. b JMJD2A knockdown in A549 cells. A549 cells were infected with indicated lentivirus for 24 h, then the mRNA and protein levels of JMJD2A were analyzed. * p < 0.05 vs. shCtrl. n = 3 in each group. c , d JMJD2A knockdown blocks KLF8 effects on the expression of P21 and CDK4 . A549 cells were infected with indicated lentivirus for 24 h, then the mRNA and protein level of P21 ( c ) and CDK4 ( d ) were analyzed. ** p < 0.01. n = 3 in each group. e JMJD2A knockdown blocks KLF8 effects on cell cycle. A549 cells were infected with indicated lentivirus for 24 h, then the cell cycle was analyzed. * p < 0.05 vs. Ctrl. f , g A549 cells were infected with indicated lentivirus and selected with puromycin and then the transduced cells were subjected to cell proliferation ( f ) and colony formation assay ( g ). * p < 0.05 vs. Ctrl. ## p < 0.01 vs. Lenti- KLF8 . n = 3 in each group. The number of cells was normalized to the control group at day 0 (%) and the percentage of cell number was shown

Article Snippet: The promoter of human JMJD2A (− 2500 bp to + 100 bp) was cloned into the pGL3 plasmid (Addgene, #64784) to generate pGL3-JMJD2A plasmid.

Techniques: Knockdown, Infection, Expressing, Colony Assay, Control

Journal: Cancer Cell International

Article Title: KLF8 overexpression promotes the growth of human lung cancer cells by promoting the expression of JMJD2A

doi: 10.1186/s12935-019-0970-3

Figure Lengend Snippet: KLF8 promotes the expression of JMJD2A. a , b KLF8 knockdown reduces the expression of JMJD2A. A549 cells were infected with indicated lentivirus for 24 h and then the mRNA ( a ) and protein ( b ) level of JMJD2A were analyzed. ** p < 0.01 vs. shCtrl. n = 3 in each group. c , d KLF8 overexpression promotes the expression of JMJD2A . A549 cells were infected with indicated lentivirus for 24 h and then the mRNA ( a ) and protein ( b ) level of JMJD2A were analyzed. n = 3 in each group. e Chromatin-immunoprecipitation (ChIP) assay showing KLF8 binding to JMJD2A promoter. ** p < 0.01 vs. shCtrl + ChIP-IgG, ## p < 0.01 vs. shCtrl + ChIP- KLF8 . n = 3 in each group. f KLF8 knockdown reduces the promoter activity of JMJD2A . A549 cells with/without sh KLF8 transduction were transfected with pGL3- JMJD2A for 24 h, then cells were subjected to luciferase analysis. ** p < 0.01 vs. shCtrl. n = 3 in each group. g KLF8 overexpression increases the promoter activity of JMJD2A. A549 cells with/without KLF8 transduction were transfected with pGL3-JMJD2A for 24 h, then cells were subjected to luciferase analysis. ** p < 0.01 vs . Lenti-Ctrl. n = 3 in each group

Article Snippet: The promoter of human JMJD2A (− 2500 bp to + 100 bp) was cloned into the pGL3 plasmid (Addgene, #64784) to generate pGL3-JMJD2A plasmid.

Techniques: Expressing, Knockdown, Infection, Over Expression, Chromatin Immunoprecipitation, Binding Assay, Activity Assay, Transduction, Transfection, Luciferase